期刊： BIOTECHNIQUES, 2021; 71 (2)
The development of a quick, single-step cloning system for generation of multiexon gene expression constructs is presented. The system allows efficien......
期刊： BIOTECHNIQUES, 2021; 71 (1)
Method summary The LMTIA technique for isothermal amplification of nucleic acids in vitro was developed in this study. The existing software for PCR d......
期刊： BIOTECHNIQUES, 2021; 70 (2)
Transcription activator-like effector (TALE) nucleases (TALENs) efficiently recognize and cleave DNA in a sequence-dependent manner. However, current ......
期刊： BIOTECHNIQUES, 2020; 68 (5)
The application of RNAi promotes the development of novel approaches toward plant protection in a sustainable way. Genetically modified crops expressi......
期刊： BIOTECHNIQUES, 2020; 68 (5)
Extracellular vesicles (EVs) are small membranous particles that contribute to intercellular communications. Separating EVs from tissue is still a tec......
期刊： BIOTECHNIQUES, 2020; 68 (5)
The establishment of transgenic plants has greatly promoted the progress of plant research. However, traditional selection methods using antibiotics o......
期刊： BIOTECHNIQUES, 2020; 69 (1)
A topologically associated domain (TAD) is a self-interacting genomic block. Detection of TAD boundaries on Hi-C contact matrix is one of the most imp......
期刊： BIOTECHNIQUES, 2020; 69 (1)
Refolding of human interleukin 17A (IL-17A) has been reported; however, the key refolding protocol was not robust enough to deliver consistent results......
期刊： BIOTECHNIQUES, 2020; 69 (1)
Mesenchymal stem cells (MSCs) represent a promising therapeutic candidate for treating many diseases. However, their proliferation and therapeutic abi......
期刊： BIOTECHNIQUES, 2020; 69 (2)
The outbreak of viral pneumonia caused by the novel coronavirus SARS-CoV-2 that began in December 2019 caused high mortality. It has been suggested th......
期刊： BIOTECHNIQUES, 2020; 69 (2)
Fluorophores SYTO 9 and propidium iodide (PI) are extensively applied in medicine, food industry and environmental monitoring to assess the viability ......
期刊： BIOTECHNIQUES, 2020; 68 (1)
The use of avidin or streptavidin in the purification of biotinylated proteins has been highly restricted due to the harsh and denaturing elution cond......
期刊： BIOTECHNIQUES, 2020; 68 (1)
Many researchers have switched to purchasing their desired plasmids from commercial suppliers to save time and resources, as we did for 17 high-risk h......
期刊： BIOTECHNIQUES, 2020; 68 (2)
Hi-C has been predominately used to study the genome-wide interactions of genomes. In Hi-C experiments, it is believed that biases originating from di......
期刊： BIOTECHNIQUES, 2020; 68 (3)
Millions of museum specimens are integral to biodiversity studies; however, DNA degradation may limit the ability to obtain DNA sequences. In this stu......
