A simple method for non-denaturing purification of biotin-tagged proteins through competitive elution with free biotin

Lin, K; Yan, Q; Mitchell, A; Funk, N; Lu, C; Xiao, H

Xiao, H (corresponding author), ImmuneChem Pharmaceut Inc, Burnaby, BC V5J 3J1, Canada.; Xiao, H (corresponding author), Gaungxi Univ, Fac Anim Sci & Technol, Nanning 530015, Guangxi, Peoples R China.

BIOTECHNIQUES, 2020; 68 (1): 41

Abstract

The use of avidin or streptavidin in the purification of biotinylated proteins has been highly restricted due to the harsh and denaturing elution cond......

Full Text Link


Links

期刊讨论 | 中国SCI论文 | 期刊主页 | 投稿经验 | 杂志官网 | 投稿链接 | 作者需知 | PMC链接 | Pubmed全文检索