Rapid Golden Gate assembly of exons from genomic DNA for protein expression in Escherichia coli and Pichia pastoris

Cheng, JH; Wu, MK; Zhong, R; Si, DY; Meng, G; Zhang, RJ; Zhang, YP

Zhang, RJ; Zhang, YP (corresponding author), China Agr Univ, Coll Vet Med, Coll Anim Sci & Technol, Lab Feed Biotechnol,State Key Lab Anim Nutr, Beijing 100193, Peoples R China.

BIOTECHNIQUES, 2021; 71 (2):

Abstract

The development of a quick, single-step cloning system for generation of multiexon gene expression constructs is presented. The system allows efficien......

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