检验

订阅

MedSci主页 / 所有栏目 / 检验

CLIN CHEM LAB MED：一种新的流式细胞术方法在常规血液学工作流程中的初步评价

2019-11-17 MedSci MedSci原创

常规血液学工作流程
关注

在高等实验室中，将血液学分析仪(HAs)获得的数据与多参数流式细胞术(FMC)获得的数据进行整合，可以提高一级筛选鉴别病理样本的特异性和敏感性。本研究的目的是对一种新的简单混合方法(HM)进行初步评价。该方法是通过将HAs试剂整合到FCM中，用碱性单克隆抗体板进行白细胞鉴别计数得到的。

在高等实验室中，将血液学分析仪(HAs)获得的数据与多参数流式细胞术(FMC)获得的数据进行整合，可以提高一级筛选鉴别病理样本的特异性和敏感性。本研究的目的是对一种新的简单混合方法(HM)进行初步评价。该方法是通过将HAs试剂整合到FCM中，用碱性单克隆抗体板进行白细胞鉴别计数得到的。

研究人员采用XN-module和CyFlow Space System对采集于K3EDTA管中的81份外周血进行分析，使用标准MoAbs和HM法分析，并使用光学显微镜(OM)进行分析。使用正常样本进行运行内不精确性分析，评估结转，并使用Passing-Bablok回归和Bland-Altman图进行数据比较。

HM方法的运行内不精确性范围在中性粒细胞(NE)为1.4%，在单核细胞(MO)为10.1%，等于或低于OM。HM法与OM法的比较显示，淋巴细胞(LY)的-巴布洛克回归斜率为0.83，MO为1.14，而NE为- 0.18，LY为0.25。布兰德-阿尔特曼相对偏倚在- 12.43% (NE)和19.77%(嗜酸性粒细胞)之间。所有11例病理标本中，两种方法的符合率均为100%。

这种新的混合方法催生了一种适合常规临床使用的白细胞鉴别计数方法，它还有助于识别形态学异常，从而降低了成本，并改进了一级血液学工作流程的筛选。

原始出处：

Michela Seghezzi，Valentina Moioli，Preliminary evaluation of a new flow cytometry method for the routine hematology workflow

本文系williamhill asia 医学（MedSci）原创编译整理，转载需授权！


评论区 (5)
#插入话题
  1. [GetPortalCommentsPageByObjectIdResponse(id=2041942, encodeId=273620419429f, content=<a href='/topic/show?id=d8684e896de' target=_blank style='color:#2F92EE;'>#工作流程#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=91, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=47896, encryptionId=d8684e896de, topicName=工作流程)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=21015, createdName=heli0118, createdTime=Sun Nov 24 17:28:00 CST 2019, time=2019-11-24, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1444596, encodeId=350d14445960a, content=<a href='/topic/show?id=3d9291459d1' target=_blank style='color:#2F92EE;'>#评价#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=87, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=91459, encryptionId=3d9291459d1, topicName=评价)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=1fee5134542, createdName=huagfeg, createdTime=Tue Nov 19 12:28:00 CST 2019, time=2019-11-19, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1466319, encodeId=56ac14663195f, content=<a href='/topic/show?id=765c8925904' target=_blank style='color:#2F92EE;'>#血液学#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=146, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=89259, encryptionId=765c8925904, topicName=血液学)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=67c86798320, createdName=hxq78318, createdTime=Tue Nov 19 12:28:00 CST 2019, time=2019-11-19, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1509974, encodeId=9cb915099e43a, content=<a href='/topic/show?id=d7716530469' target=_blank style='color:#2F92EE;'>#流式细胞术#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=97, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=65304, encryptionId=d7716530469, topicName=流式细胞术)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=7cd410182644, createdName=w984448tii, createdTime=Tue Nov 19 12:28:00 CST 2019, time=2019-11-19, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1610138, encodeId=ff9e161013820, content=<a href='/topic/show?id=eb6b1145939' target=_blank style='color:#2F92EE;'>#Med#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=93, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=11459, encryptionId=eb6b1145939, topicName=Med)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=552a19396740, createdName=ms3994565386320060, createdTime=Tue Nov 19 12:28:00 CST 2019, time=2019-11-19, status=1, ipAttribution=)]
    2019-11-24 heli0118

    #工作流程#

    0

  2. [GetPortalCommentsPageByObjectIdResponse(id=2041942, encodeId=273620419429f, content=<a href='/topic/show?id=d8684e896de' target=_blank style='color:#2F92EE;'>#工作流程#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=91, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=47896, encryptionId=d8684e896de, topicName=工作流程)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=21015, createdName=heli0118, createdTime=Sun Nov 24 17:28:00 CST 2019, time=2019-11-24, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1444596, encodeId=350d14445960a, content=<a href='/topic/show?id=3d9291459d1' target=_blank style='color:#2F92EE;'>#评价#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=87, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=91459, encryptionId=3d9291459d1, topicName=评价)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=1fee5134542, createdName=huagfeg, createdTime=Tue Nov 19 12:28:00 CST 2019, time=2019-11-19, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1466319, encodeId=56ac14663195f, content=<a href='/topic/show?id=765c8925904' target=_blank style='color:#2F92EE;'>#血液学#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=146, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=89259, encryptionId=765c8925904, topicName=血液学)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=67c86798320, createdName=hxq78318, createdTime=Tue Nov 19 12:28:00 CST 2019, time=2019-11-19, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1509974, encodeId=9cb915099e43a, content=<a href='/topic/show?id=d7716530469' target=_blank style='color:#2F92EE;'>#流式细胞术#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=97, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=65304, encryptionId=d7716530469, topicName=流式细胞术)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=7cd410182644, createdName=w984448tii, createdTime=Tue Nov 19 12:28:00 CST 2019, time=2019-11-19, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1610138, encodeId=ff9e161013820, content=<a href='/topic/show?id=eb6b1145939' target=_blank style='color:#2F92EE;'>#Med#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=93, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=11459, encryptionId=eb6b1145939, topicName=Med)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=552a19396740, createdName=ms3994565386320060, createdTime=Tue Nov 19 12:28:00 CST 2019, time=2019-11-19, status=1, ipAttribution=)]
    2019-11-19 huagfeg

    #评价#

    0

  3. [GetPortalCommentsPageByObjectIdResponse(id=2041942, encodeId=273620419429f, content=<a href='/topic/show?id=d8684e896de' target=_blank style='color:#2F92EE;'>#工作流程#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=91, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=47896, encryptionId=d8684e896de, topicName=工作流程)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=21015, createdName=heli0118, createdTime=Sun Nov 24 17:28:00 CST 2019, time=2019-11-24, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1444596, encodeId=350d14445960a, content=<a href='/topic/show?id=3d9291459d1' target=_blank style='color:#2F92EE;'>#评价#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=87, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=91459, encryptionId=3d9291459d1, topicName=评价)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=1fee5134542, createdName=huagfeg, createdTime=Tue Nov 19 12:28:00 CST 2019, time=2019-11-19, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1466319, encodeId=56ac14663195f, content=<a href='/topic/show?id=765c8925904' target=_blank style='color:#2F92EE;'>#血液学#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=146, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=89259, encryptionId=765c8925904, topicName=血液学)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=67c86798320, createdName=hxq78318, createdTime=Tue Nov 19 12:28:00 CST 2019, time=2019-11-19, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1509974, encodeId=9cb915099e43a, content=<a href='/topic/show?id=d7716530469' target=_blank style='color:#2F92EE;'>#流式细胞术#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=97, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=65304, encryptionId=d7716530469, topicName=流式细胞术)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=7cd410182644, createdName=w984448tii, createdTime=Tue Nov 19 12:28:00 CST 2019, time=2019-11-19, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1610138, encodeId=ff9e161013820, content=<a href='/topic/show?id=eb6b1145939' target=_blank style='color:#2F92EE;'>#Med#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=93, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=11459, encryptionId=eb6b1145939, topicName=Med)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=552a19396740, createdName=ms3994565386320060, createdTime=Tue Nov 19 12:28:00 CST 2019, time=2019-11-19, status=1, ipAttribution=)]
    2019-11-19 hxq78318

    #血液学#

    0

  4. [GetPortalCommentsPageByObjectIdResponse(id=2041942, encodeId=273620419429f, content=<a href='/topic/show?id=d8684e896de' target=_blank style='color:#2F92EE;'>#工作流程#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=91, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=47896, encryptionId=d8684e896de, topicName=工作流程)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=21015, createdName=heli0118, createdTime=Sun Nov 24 17:28:00 CST 2019, time=2019-11-24, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1444596, encodeId=350d14445960a, content=<a href='/topic/show?id=3d9291459d1' target=_blank style='color:#2F92EE;'>#评价#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=87, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=91459, encryptionId=3d9291459d1, topicName=评价)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=1fee5134542, createdName=huagfeg, createdTime=Tue Nov 19 12:28:00 CST 2019, time=2019-11-19, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1466319, encodeId=56ac14663195f, content=<a href='/topic/show?id=765c8925904' target=_blank style='color:#2F92EE;'>#血液学#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=146, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=89259, encryptionId=765c8925904, topicName=血液学)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=67c86798320, createdName=hxq78318, createdTime=Tue Nov 19 12:28:00 CST 2019, time=2019-11-19, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1509974, encodeId=9cb915099e43a, content=<a href='/topic/show?id=d7716530469' target=_blank style='color:#2F92EE;'>#流式细胞术#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=97, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=65304, encryptionId=d7716530469, topicName=流式细胞术)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=7cd410182644, createdName=w984448tii, createdTime=Tue Nov 19 12:28:00 CST 2019, time=2019-11-19, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1610138, encodeId=ff9e161013820, content=<a href='/topic/show?id=eb6b1145939' target=_blank style='color:#2F92EE;'>#Med#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=93, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=11459, encryptionId=eb6b1145939, topicName=Med)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=552a19396740, createdName=ms3994565386320060, createdTime=Tue Nov 19 12:28:00 CST 2019, time=2019-11-19, status=1, ipAttribution=)]
    2019-11-19 w984448tii

    #流式细胞术#

    0

  5. [GetPortalCommentsPageByObjectIdResponse(id=2041942, encodeId=273620419429f, content=<a href='/topic/show?id=d8684e896de' target=_blank style='color:#2F92EE;'>#工作流程#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=91, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=47896, encryptionId=d8684e896de, topicName=工作流程)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=21015, createdName=heli0118, createdTime=Sun Nov 24 17:28:00 CST 2019, time=2019-11-24, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1444596, encodeId=350d14445960a, content=<a href='/topic/show?id=3d9291459d1' target=_blank style='color:#2F92EE;'>#评价#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=87, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=91459, encryptionId=3d9291459d1, topicName=评价)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=1fee5134542, createdName=huagfeg, createdTime=Tue Nov 19 12:28:00 CST 2019, time=2019-11-19, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1466319, encodeId=56ac14663195f, content=<a href='/topic/show?id=765c8925904' target=_blank style='color:#2F92EE;'>#血液学#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=146, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=89259, encryptionId=765c8925904, topicName=血液学)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=67c86798320, createdName=hxq78318, createdTime=Tue Nov 19 12:28:00 CST 2019, time=2019-11-19, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1509974, encodeId=9cb915099e43a, content=<a href='/topic/show?id=d7716530469' target=_blank style='color:#2F92EE;'>#流式细胞术#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=97, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=65304, encryptionId=d7716530469, topicName=流式细胞术)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=7cd410182644, createdName=w984448tii, createdTime=Tue Nov 19 12:28:00 CST 2019, time=2019-11-19, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1610138, encodeId=ff9e161013820, content=<a href='/topic/show?id=eb6b1145939' target=_blank style='color:#2F92EE;'>#Med#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=93, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=11459, encryptionId=eb6b1145939, topicName=Med)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=552a19396740, createdName=ms3994565386320060, createdTime=Tue Nov 19 12:28:00 CST 2019, time=2019-11-19, status=1, ipAttribution=)]
    2019-11-19 ms3994565386320060

    #Med#

    0

相关威廉亚洲官网

Hypertension：常规和动态高血压的患病率、治疗和控制率分析！

总之，在所有检查的队列中高血压的高患病率表明需要改进其预防、治疗和控制。高血压管理的策略应继续不仅着眼于预防和可改变的危险因素，也应考虑社会问题。

讨论：williamhill asia 到底该遵循患者的意愿还是医疗的常规？

williamhill asia 妇产科的主任郎景和教授说，一个手术的成功，75%在于决策，25%在于技术。 对于治疗方式的选择，的确，作为医生和患者都是需要再三考虑和斟酌。

Clin Oral Investig：选择常规还是牙周再生手术疗法：一项对2种牙周期刊发表文章的比较

这篇研究的目的是为了比较北美和欧洲2本主要牙周期刊在1982/1983和2012/2013年间刊出的关于常规或牙周再生手术文章的数量和内容。